关键词: 环状 RNA, 胶质瘤, 细胞增殖

Abstract: Objective To explore the function and mechanism of circular RNA circ-VCAN in glioma. Methods The expression of circular RNA circ-VCAN in normal glial cells and glioma cells was confirmed by fluorescence quantitative PCR. Nucleocytoplasmic separationexperiments verified the distribution of circ- VCAN in the nuclear or cytoplasmic. RNaseR linear RNA digestion experiment was used to examine the circ- VCAN resistance to digestion of RNaseR. Circ- VCAN knockdown transfected cell lines were constructed. CCK8 was used to detect the cell proliferation ability after knockdown the circ- VCAN.Western blot was used to detect the expression levels of the NF- κB pathway proteins. Results When compared to the normal glial cells（HA1800）Circular RNA circ- VCAN was highly expressed in glioma cells，the differences were statistically significant（P<0.05）. Nucleocytoplasmic separation experiments demonstrated that circ-VCAN mainly existed in the cytoplasm. Circ-VCAN could resist digestion of RNaseR. Knock down circ- VCAN could inhibit the proliferation of U373 and T98G cells（P<0.05）. Western blot analysis showed that knockdown of circ-VCAN in U373 and T98G cells decreased expression of NF- κB pathway protein P- P65. Conclusion Knockdown circ- VCAN in the glioma cells can slow down the growth of glioma cells，suggesting that circ-VCAN may become a new therapeutic target.

Key words: circular RNA, glioma cell, proliferation