关键词: 短肠综合征, 结肠瓣膜成形术, Wnt3a, β-catenin, 上皮细胞

Abstract: Objective To investigate the protective effect and potential mechanism of colonic flap reconstruction on colonic tissue in rats with short bowel syndrome （SBS） by upregulating Wnt3a and β-catenin expression. Methods Rats were randomly divided into sham operation （Sham）, SBS model （Model）, and colonic flap reconstruction treatment （Surgery） groups, with 10 rats in each group. Serum Wnt3a levels were detected by ELISA. The mRNA and protein expression of Wnt3a and β-catenin in colonic tissue were analyzed by qPCR and Western blot. Immunohistochemistry was used to observe the expression and distribution of α-amylase and bile salt transport protein in proximal and distal epithelial cells of the colonic flap. Immunohistochemistry was also used to detect the expression and localization of Wnt3a, β-catenin, α-amylase, and bile salt transport protein in colonic tissue. Results Compared with the Sham group, there was no significant difference in serum Wnt3a levels in the Model group （P>0.05）, while Wnt3a levels in the Surgery group were significantly higher than those in the Sham and Model groups （P<0.05）. Compared with the Sham group, the mRNA and protein expression of Wnt3a and β-catenin in colonic tissue were significantly downregulated in the Model group （P<0.05）. The expression of Wnt3a and β-catenin in the Surgery group was significantly higher than that in the Model group （P<0.05）. Immunohistochemical staining showed that there were differences in the expression and distribution of α-amylase and bile salt transport protein in proximal and distal epithelial cells of the colonic flap. Conclusion Colonic flap reconstruction can alleviate pathological changes in SBS rats to a certain extent by upregulating the expression of Wnt3a and β-catenin, which may be one of the potential mechanisms of its therapeutic effect.

Key words: short bowel syndrome, colonic flap reconstruction, Wnt3a, β-catenin, epithelial cells