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    20 April 2017, Volume 17 Issue 02
    Expression profiles of long noncoding RNA in advanced glycosylation end products treated dermal fibroblast
    HU Mengdie,ZHOU Liyan,WANG Xiaoyi,WANG Wei,REN Meng,YAN Li.
    2017, 17(02):  135-139.  DOI: 10.3969/j.issn.1009-976X.2017.02.001
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    Objective   To explore the expression profile variation of long noncoding RNA (IncRNA) in advanced glycosylation end products (AGEs) treated dermal fibroblast compared with normal dermal fibroblast. Methods The differences of lncRNA expression profile between AGEs treated fibroblast and normal fibroblast were analyzed through lncRNA microarray. LncRNA with differential expression were screened out after pretreatment of raw data. Results Compared with normal fibroblast, 3421 lncRNAs were identified to be different expression (fold change≥2.0)in AGEs treated fibroblast. 1079 were up?regulated and 2342 were down?regulated. 448 lncRNAs (208 up?regulated and 240 down?regulated ) were highly differentially expressed with absolute fold change greater than ten. Conclusion      The expression profile of lncRNA in AGEs treated fibroblast was significantly changed in comparsion with normal fibroblast. These lncRNA may be involved in the occurrence and development of diabetic dermopathy. 

    Effect of bone marrow?derived mesenchymal stem cells on autophagy and apoptosis of human coronary artery endothelial cells under hypoxia condition
    MA Xun,LI Hongmu,WANG Lueli,TAO Jun,LIN Xifeng,HUA Ping,XIONG Lihua.
    2017, 17(02):  140-146.  DOI: 10.3969/j.issn.1009-976X.2017.02.002
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    Objective To investigate whether mesenchymal stem cells lead to induction of autophagy and change at early apoptotic rate under hypoxia condition on coronary artery endothelial cells. Methods We constructed the co?culture model of HCAECs(human coronary artery endothelial cells) and hBMMSCs(human bone marrow?derived mesenchymal stem cells). In experiment group,HCAEC co?cultured with MSC,HCAEC cultured alone in blank group. And in control group ,HCAEC cultured alone using same medium with experiment group. Each group separately cultured 24 h under nomoxia (5% CO2 ,95% air)and hypoxia(1% O2 ,5% CO2 ,94% N2)condition. The HCAEC′s expression of LC3 and Beclin?1 at mRNA and protein level was detected by Real?Time PCR and Western blotting , respectively. Flow cytometry was used to detect early apoptotic rate. Results The protein expression of LC3 and Beclin?1 in co?culture groups and hypoxia groups significantly higher than separate culture groups and nomoxia groups respectively(P<0.05),while mRNA expression of LC3 and Beclin?1 in each group shows no difference (P>0.05). The early apoptotic rate of co?culture group under hypoxia was 8.86%±1.48%,which was significantly lower than those in separate culture group under hypoxia and control group under hypoxia(15.23%±2.56% and 14.49%±3.31% ,P<0.05). Conclusion MSC induces autophagy in HCAEC,along with decrease of early apoptosis rate of HCAEC under hypoxia condition.

    Clinical diagnosis and treatment experience of endovascular therapy on acute superior mesenteric artery ischemia
    HUANG Kai,CHEN Qinchang,LIN Xixia,LI Lingling,LI Honghao.
    2017, 17(02):  147-149.  DOI: 10.3969/j.issn.1009-976X.2017.02.003
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    Objective To summarize the effect of endovascular therapy of acute superior mesenteric artery ischemia(ASMAI). Methods From May 2015 to June 2016,7 patients suffered from ASMAI diagnosed via CT angiography (CTA),underwent endovascular therapy. Results One patient with severe symptoms for more than 24 hours needed to accept partial small bowel resection after endovascular therapy. Another one case accompanied with retroperitoneal hematoma during the endovascular thrombolytic therapy. However, after two months of conservative treatment, the retroperitoneal hematoma significantly was relieved,and the compensatory circulation occurred. And the rest of all the cases(71.42%)were successfully achieved via endovascular therapy,whom presented with the clinical symptoms obviously alleviated,and no one died of the disease. Conclusion The timely application of endovascular technique in the treatment of ASMAI can be to the benefit of patients′disability rate and morbidity.

    An investigation of volume administration guided by stroke volume variation in hepatolobectomy
    WENG Hanqin,CHEN Xiaohong,HAN Qi,SUN Hongwu.
    2017, 17(02):  150-154.  DOI: 10.3969/j.issn.1009-976X.2017.02.004
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    Objective To investigate the effect of stroke volume variation (SVV)as an index of volume administration in hepatolobectomy. Methods Sixty patients undergoing hepatolobectomy were assigned to two groups,the observation group(Group S,CVP weas maintained at 0-5 cm H2O and SVV maintained at 10±2%)and the control group (Group C ,CVP was maintained at 0-5 cm H2O). The hemodynamics of two groups of patients were recorded at certain time points ,including the 30 minutes after anesthesia(T1),the end time of hepatectomy(T2),and the end time of operation(T3). The hemo? globin (Hb),blood cell volume (HCT),creatinine (Cr) and urea nitrogen (Bun) levels of the two groups were recorded the day before the operation(A),the first day after hepatectomy(B)and the day after the operation(C). The operation time,amount of bleeding and urine,the recovery time of gastrointes? tinal function,the length of hospital stay and the incidence rate of complications of the two groups were recorded. Results There were no significant differences in gender,age,body weight and liver function between the two groups. The comparison of blood flow dynamics of the two groups of patients at T1 ,T2 and T3 had no statistically significant difference in Group S patients. There was no significant difference in  invasive blood pressure(ABP)and central venous pressure(CVP)in Group C patient;there was significant difference in cardiac output(CO)and heart rate(HR)T2,T3 and T1 comparisons(P<0.05). There was no significant difference in the preoperative and postoperative laboratory indexes of Group S(P>0.05). There was no significant difference in the level of urea nitrogen(P>0.05);there was significant difference in hemoglobin,blood cell pressure and creatinine levels in Group C patients(P<0.05). The clinical data of the two groups showed little change in urine volume;the liver cutting time,blood loss,intraoperative fluid,gastrointestinal recovery time,hospitalization time and complications showed significant difference in the incidence rate(P<0.05). Conclusion Liver resection operation under SVV dynamic guidance capacity management is conducive to the control of intraoperative bleeding and reduction blood loss, shortens the operation time,optimizes the individualized rehydration.

    Predictive value of procalcitonin and C?reactive protein in the severe acute pancreatitis
    ZHANG Xinchang,ZENG Yanmei,LIU Yue,TANG Jianhui.
    2017, 17(02):  154-157.  DOI: 10.3969/j.issn.1009-976X.2017.02.005
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    explore the predictive value of procalcitonin Pthe severe acute pancreatitis ptregression and ROC curve were utilized to analysis the correlation between PCTand the risk of severe acute pancreatitis within 24 hours of onset. Results The mean value of PCT and CRP in the observation group was significantly higher than control groupObjective To explore the predictive value of procalcitonin (PCT) and C?reactive protein (CRP)in the severe acute pancreatitis (SAP). Method A retrospective study in 68 cases of acute pancreatitis patients who accepted treatment in our hospital from Jan. 2013 to Dec. 2015 were conducted. All cases were divided into mild pancreatitis group (control group) and severe acute pancreatitis group (observation group) according to the 2013 edition of Chinese Acute Pancreatitis Diagnosis and Treatment Guideline. T?test,Logistic regression and ROC curve were utilized to analysis the correlation between PCT,CRP and the risk of severe acute pancreatitis within 24 hours of onset. Results The mean value of PCT and CRP in the observation group was significantly higher than control group(P< 0.001). By logistic regression analysis,the OR values of PCT and CRP were 1.41,1.125 respectively. In both PCT and CRP,the confidence interval of the OR value of RP 95% was greater than one. Thus,both PCT and CRP were the risk factors for SAP and they could be combined to predict the risk of SAP. Furthermore,the ROC curves indicated that PCT was superior to CRP in sensitivity and accuracy for predicting the risk of SAP. Conclusion Early detection of serum PCT and CRP level could reflect the severity of AP patients,and PCT was more accurate and sensitive than CRP. Moreover,combination of PCT and CRP could predict the risk of SAP and provided valuable guidance for the SAP treatment.

    The application of total laparoscopy in radical gastrectomy with π?shaped esophagojejunal anas? tomosis
    LIAO Ziqun,CHEN Weirong,CHEN Xigui,CAI Gaoyang,DENG Xi.
    2017, 17(02):  158-161.  DOI: 10.3969/j.issn.1009-976X.2017.02.006
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    Objective To evaluate the safety and efficacy of total laparoscopy in radical gastrectomy. Methods A retrospective study was done on clinical data and follow up of 8 cases treated with laparoscopic radical gastrectomy with π?shaped esophagojejunal anastomosis. Results Total laparoscopy in radical gastrectomy was successfully performed for 8 patients. No case was converted to open surgery. No intraoperative death occurred. The mean operation time, blood loss, postoperative hospital stay were (292±32)min, (75±35)ml, (15±2)days, respectively. There was no postoperative complications. All patients were followed up for 3 to 12 months. No local recurrence or distant metastase was found. No car? cinoma was implanted in the trocar holes. Conclusions Total laparoscopy in radical gastrectomy is feasible and safe and has a favorable short term outcome.

    The clinical application of 3D rotatable laparoscopic anterior resection for rectal cancer
    GENG Yan,JU Yongle,LU Guangsheng,REN Baojun,ZHANG Yanxiang,FENG Jing.
    2017, 17(02):  162-165.  DOI: 10.3969/j.issn.1009-976X.2017.02.007
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    Objective To summarize the experience and feasibility of three?dimensional(3D) rotatable laparoscopic anterior resection for rectal cancer and compare with two?dimensional(2D)lapa? roscopy. Methods We retrospectively analyzed 86 cases(from Oct. 2014 to Sep. 2016)of rectal cancer for laparoscopy(40 cases for 3D group and 46 cases for 2D group). We compared the mean of operation duration, intraoperative blood loss, postoperative exhaust time, postoperative hospital stay, number of lymph node dissection between two groups. Results All cases were performed with laparoscopic procedure. As compared to 2D group, 3D group had less blood loss(35.0±16.8 ml vs. 44.3±18.2 ml, t=-2.449, P=0.016). No significant differences were seen in operative time(126.2±24.2 min vs. 131.8±30.2 min, t=-0.939, P=0.350), postoperative exhaust time(2.7±1.2 d vs. 2.8±0.9 d, t=-0.441, P=0.661), postoperative hospital stay (8.4±2.6 d vs. 8.6±2.5 d, t=- 0.363, P=0.717), number of lymph node dissection(16.4±3.4 vs. 15.8±5.2, t=0.623, P=0.535)between two groups. Conclusions It is safe to 3D rotatable laparoscopic anterior resection for rectal cancer. Compared with 2D laparoscopy, 3D rotatable laparoscopy is same in flexibility of operation, but it can give us more accurate space orientation, more distinct anatomical layer and more exquisite separation.

    Effects of the recombinant bovine basic fibroblast growth factor gel on anal fistula wound,angiogenesis,and expression of VEGF and VEGFR?2
    XIE Jialiang,NIE Honghai,ZHU Weizhou.
    2017, 17(02):  166-168.  DOI: 10.3969/j.issn.1009-976X.2017.02.008
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    Objective To investigate the effects of shellfish Fuxin gel(recombinant bovine basic fibroblast growth factor gel)on anal fistula wound,angiogenesis and vascular endothelial growth factor(VEGF)and its receptor(VEGFR?2)expression. Methods Sixty cases of post?operation of anal fistula thread?drawing from Jan 2013 to Dec 2016 were selected and divided into observation group (n=30), treated with Bei Fuxin Gel,and control group (n=30),treated with Vaseline gauze. The number of capillaries and the expression level of VEGF and VEGFR?2 were detected in 7 days and 14 days after treatment. Results The number of capillaries,expression level of VEGF and VEGFR?2 of the observation group are much higher,with statistically significance(all P values <0.05)respectively. Related analysis showed that wound capillary number and VEGF expression was significantly positive correlated(r=0.714, P<0.05), wound capillary number and VEGFR?2 expression was significantly positive correlation (r=0.652,P<0.05). Conclusion Bei Fuxin Gel can promote angiogenesis on the wound after anal fistula surgery,accelerate the expression of VEGF and VEGFR?2. There was a significant positive correlation between the number of capillaries and VEGF,VEGFR?2 expression.

    Transvaginal extraction of the specimen in laparoscopic sigmoidectomy or protectomy combined with total hysterectomy and bilateral adnexectomy for local advanced colorectal cancer
    YU Si, DENG Jianzhong,JI Yong,LIN Yiban,WU Qiaoling,ZHU Jiacheng.
    2017, 17(02):  169-173.  DOI: 10.3969/j.issn.1009-976X.2017.02.009
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    Objective To investigate the safety and feasibility of transvaginal extraction of the specimen in laparoscopic sigmoidectomy or protectomy combined with total hysterectomy and bilateral adnexectomy for colorectal cancer. Methods From January 2000 to December 2014,36 cases of sigmoid colon or rectal cancer were undergone laparoscopic sigmoidectomy or protectomy combined with total hysterectomy and bilateral adnexectomy in which the specimen were extracted by vagina. Results No patients needed open conversion. Mean operative time was(173±13)min. Mean amount of blood loss was (80±12)ml. No patients needed painkillers post operation. The mean VAS score was(2.9±1.0). Mean time of anal flatus was (82±18) h. Mean time of postoperative hospital stay was(6.9±1.8)d. The rate of complication was 31%(11/36),including anastomotic fistula in one case ,vaginal stump fistula in one case ,rectalvaginal fistula in two cases ,early postoperative inflammatory intestinal obstruction in 3 cases,urinary retention in 2 cases,pulmonary infection in 2 cases. The median time of follow?up was 53 months (14 ~ 128 m). The median survival time was 63.95 months. One?year survival rate was 100%,three?year survival rate was 83% and five?year survival rate was 61%. Conclusion Transvaginal extraction of the specimen in laparoscopic sigmoidectomy or protectomy combined with total hysterectomyand bilateral adnexectomy for local advanced colorectal cancer is feasible and safe in surgical procedure.It had not only such advantages as little trauma,quick recovery,but also satisfactory therapeutic effectof oncology in long?term follow?up.

    Comparative study of open and single?incision laparoscopic preperitoneal techniques for inguinal hernia repair
    HUANG Jinlin,YU Jinlong.
    2017, 17(02):  174-182.  DOI: 10.3969/j.issn.1009-976X.2017.02.010
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    Objective To compare the clinical treatment effect of open and single?incision laparoscopic preperitoneal techniques for inguinal hernia repair. Methods The clinical data of 50 patients with unilateral inguinal hernia treated in our hospital from January 2015 to June 2015 were retrospectively analyzed. Of 50 cases,21 cases underwent single?port laparoscopic total peritoneal inguinal hernia repair(single?port laparoscopic group)and 29 cases of open tension?free hernia repair surgery(open goup). The clinical outcomes were recorded and compared between two groups,including operation time,intraoperative blood loss,postoperative hospital stay,postoperative complications,24 hours after surgery and chronic pain(more than one month),total hospital costs. Results The operation time,the amount of intraoperative blood loss,postoperative average hospitalization time and 24 hours postoperatively and were significantly lower than those in the open group and the difference (P<0.05). The total cost of hospitalization was significantly lower than that of SILS?TEP group(P<0.05). There was no significant difference in chronic pain(more than one month)and postoperative complications between the two groups (P>0.05). Conclusion Single?hole laparoscopic total intraperitoneal inguinal hernia tension?free repair of patients is superior to the open surgery but higher hospital cost.

    Construction of rabbit tissue?engineered corpus cavernosum with co?culture of BM?MSCs and CD34+ NCs and transplantation in vivo
    LI Ruiting,LIU Zhihan,JI Chenyang,XIAO Xiaolian, LIANG Weiqiang,ZHANG Jinming.
    2017, 17(02):  177-182.  DOI: 10.3969/j.issn.1009-976X.2017.02.011
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    Objective To investigate the feasibility of co?cultured mesenchymal stem cells(MSCs) and CD34 + mononuclear cells seeded in acellular matrix to construct a section of rabbit tissue?engineered cavernosum in vivo. Methods MSCs and CD34+ mononuclear cells (MNCs)were extracted from bone marrow and peripheral blood. After being identified,the two components of the cells were seeded in prepared acellular corporal matrix alongside with MSCs and CD34 + MNCs seeded in the same density respectively. The cell?laden matrix was transplanted into a rabbit model of penile interruption after culture for 4 days in vitro. Three months after implantation ,animals were killed and the engineered tissue was collected for histological examination (H&E staining and Masson′ s trichrome staining). Results The engineered corporal tissue near tunica albuginea in MSCs and CD34+ MNCs cocultured group preserved the histological feather of intact corporal tissue. The other 3 groups,including MSCs group,CD34+ MNCs group and acellular matrix group,failed to preserve the corporal structure. Instead,they underwent fibrosis and formed scar tissue though samples from MSCs group showed more small vessels than from CD34+ MNCs group and acellular matrix group (P<0.05). Conclusion Our work demonstrates the feasibility of co?implantation of MSCs and CD34+ MNCs in acellular matrix to construct corporal tissue resembling to native one. CD34+MNCs promote vascularization in vivo along with MSCs rather than alone.

    Analysis of partial tubeless percutaneous nephrolithotomy in 135 cases with upper urinary calculi
    HGUANG Rui
    2017, 17(02):  183-185.  DOI: 10.3969/j.issn.1009-976X.2017.02.012
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    Objective To discuss the feasibility,safety and efficacy of partial tubeless percutaneous nephrolithotomy (using Double?J tube instead of nephrostomy tube) in the treatment of upper urinary calculi. Methods The medical records of partial tubeless percutaneous nephrolithotomy from January 2016 and December 2016 in our hospital were collected and analyzed retrospectively including operation time,bleeding and blood transfusion during the operation ,stone?free rate ,complication pro? files ,catheter indwelling time and the length of hospital stay. Results With a total observation of the successful 135 cases,the operation time ranged from 20 to 65 minutes with a mean of 48±11 min, among which 75 cases with size F18,60 cases with size F20,127 cases with single?path and 8 cases with two?path. The success rate was 98.5% (132/135) with none severe complications. The average catheter indwelling time was 3.5±0.5 days and the average postoperative hospital stay was 4.5±0.5 days. Conclusion The effect of partial tubeless percutaneous nephrolithotomy in the treatment of upper urinary calculi is satisfactory,which is safer and fast recovered.

    Clinical observation to keep low renal pelvic pressure in single tract minimally invasive percutaneous nephrolithotomy
    LIU Weiguang,FENG Zhiping,ZHONG Donghai,ZHU Jianming.
    2017, 17(02):  186-193.  DOI: 10.3969/j.issn.1009-976X.2017.02.013
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    Objective To evaluate the method of controlling renal pelvic pressure in single tract minimally invasive percutaneous nephrolithotomy (MPCNL). Methods Eighty patients with renal stones underwent MPCNL were selected and assigned to two groups. The observation group (40 cases) was treated with real?time intraoperative monitoring of renal pelvic pressure operation ,adjustment of the perfusion fluid filling and flowing,and control of the intra?pelvic pressure,The control group(40 cases) only was recorded the intra?pelvic pressure. The perfusion fluid volume ,average renal intrapelvic pres? sure,intrapelvic pressure ≥30 mmHg cumulative time,operation time and postoperative complications were compared between the two groups. Results For the observation group and the control group,the perfusion fluid volume(L)were 14.5±3.8,26.8±6.5,the average renal intrapelvic pressure (mmHg) were 14.33±3.71,28.4±5.5,the renal intra?pelvic pressure ≥30 mmHg cumulative time(s)were 42.4± 5.7,368.4±20.7,which demonstrated significant differences (P<0.05). The average operation time (min)were 123±20,119±31,with no significant difference(P>0.05). The occurrences of postoperative pain,fever,WBS increase in the control group were significantly higher than the test group (P<0.05). Conclusion         The operation of controlling liquid perfusion volume can produce effective control of renal pelvis pressure operation in the MPCNL,which makes the operation safer.

    Synchronous treatment of percutaneous transhepatic and choledochoscopy for recurrent hepatoli? thiasis
    LIN Shuwen Lin,FANG Yinghua,DING Xinming,YUAN Zhiming,WAN Lipeng,JI Chenggang
    2017, 17(02):  189-193.  DOI: 10.3969/j.issn.1009-976X.2017.02.014
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    Objective To Summarize our experience on percutaneous transhepatic one?stage lithotomy with choledochoscope in treatment of recurrent hepatolithiasis. Methods A retrospective analysis of clinical data in 22 cases with recurrent hepatolithiasis in our hospital from October 2014 to June 2016 was performed. The patients were treated by direct puncture of hepatic duct under B?ultrasound guidance to establish a percutaneous tract ,and then lithotomy with choledochoscope for extraction of the bile duct stones was performed contemporaneously. Results Twenty?one patients were successful to be extracted stone,only one patient was converted to laparotomy. Of 21 cases,17 were healed in one?time stone removal,three underwent two?time operation,one received three?time operation. The main complication was hemobilia and was treated conservatively ,and no serious complications and death. No recur?rence was found in all patients followed up until now. Conclusion In properly selected patients of recurrent hepatolithiasis,synchronous treatment of percutaneous transhepatic and choledochoscopy is feasible,effective and safe.

    Application of soft?tissue expander in implant reconstruction for breast cancer
    ZHANG Xiao,YE Yifeng
    2017, 17(02):  194-197.  DOI: 10.3969/j.issn.1009-976X.2017.02.015
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    Objective     To summarize the clinical experience of breast reconstruction with soft tissue expander and delayed implant?based reconstruction. Methods Between February 2011 and April 2016,41 patients with breast cancer underwent soft?tissue expander and delayed implant?based reconstruction. The age ranged from 28 to 58 years(median,42 years). Thirty?eight cases revealed invasive ductal carcinoma and 3 cases invasive ductal carcinoma+DCIS. The median diameter was 2.4 cm,ranging from 1.0 to 4.5 cm. Twenty?four cases were rated as stage Ⅰ ,nine cases as stage ⅡA,seven cases as stage ⅡB,and one case as stage ⅢA. Thirty?two cases underwent modified radical mastectomy and nine cases underwent nipple?sparing mastectomy ,and followed by immediate soft?tissue expander implanta? tion. All the patients received adjuvant therapy according to the NCCN guideline. Delayed implant reconstruction was performed on completing the radiotherapy. Results Thirty?eight cases were successfully reconstructed. There were ten cases of complications out of 41 cases,including five cases of infection , one case of local skin necrosis ,one case of incision dehiscence ,one case ischemia of skin flap and two cases ischemia of nipple?areola complex. At six months after reconstruction ,the aesthetic Results were excellent in 19 cases,good in 15 cases,fair in two cases and poor in one case. All the cases were follow ?up for 6~56 months(median ,26 months),and three cases of metastasis were found and there is no local recurrence. Conclusion Soft?tissue expander implantation with delayed implant reconstruction can effectively improve the cosmetic effect for patients with breast cancer,without increasing the risk of metastasis and complications.

    Effect of postoperative adjuvant chemotherapy on prognosis of elderly breast cancer
    WANG Yongnan,LI Wenping,YU Haiyun,WAN Jian
    2017, 17(02):  198-201.  DOI: 10.3969/j.issn.1009-976X.2017.02.016
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      Objective To explore the effect of postoperative adjuvant chemotherapy on progno? sis of elderly breast cancer. Methods The data of 80 patients with breast cancer who were hospitalized for Ⅰ~ⅢA ≥65 years of age from Jan. 2003 to Dec. 2013 were analyzed. Of 80 cases,47 cases received adjuvant chemotherapy , 33 cases did not received adjuvant chemotherapy. The clinicopathological features and clinical treatment characteristics were analyzed. Results Compared with patients who not received postoperative adjuvant chemotherapy ,patients received adjuvant chemotherapy were younger (P=0.005),with fewer comorbidities (P=0.040),higher rates of axillary lymph node metastasis (P< 0.001),more the rate of ER/PR negative(P=0.029),more the rate of receiving radiotherapy(P=0.005). There were no significant differences in tumor histological grade ,tumor size,HER2 expression,operation and endocrine therapy(P>0.05). The median follow?up 73 months,between adjuvant chemotherapy group and non?adjuvant chemotherapy group ,in which disease?free survival( DFS )had no signifi? cant difference (78.7% vs 90.9% ,P=0.147),overall survival(OS)also was not significantly different(83.0% vs 93.9%,P=0.098). Conclusions The benefit for elderly patients with postoperative adjuvant chemotherapy for breast cancer is not obvious. But for those in younger ,less complications asso? ciated with axillary lymph node metastasis ,ER/PR negative,the patients should be comprehensively as? sessed for the tolerance and benefit before postoperative adjuvant chemotherapy.

    Experience and skill of methylene blue in sentinel lymph nodes localization of cN0 thyroid papil?lary carcinoma
    HE Dongtian,GUAN Miaozhu,LI Chao,LI Weiwen,SU Yaorong,JIANG Wenchuan.
    2017, 17(02):  201-204.  DOI: 10.3969/j.issn.1009-976X.2017.02.017
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    Objective To summarize up the experience in applying methylene blue dye to locate sentinel lymph nodes of cN0 thyroid papillary carcinoma. Mehtods Fifty?one patients of thyroid papillary carcinoma were performed sentinel lymph node biopsy by using methylene blue dye positioning. After Functional neck dissection ,the number of sentinel lymph nodes and cervical lymph nodes were recorded and analyzed. Results Among the 51 patients,46 cases were found to have blue?stain sentinel lymph nodes with a detection rate of 90.2% ,positive rate of 45.7% ,negative rate of 50% and false negative rate of 4.3%. The sensitivity was 91.3% (21/23),and the accuracy was 95.7% (44/46). Conclusion Methylene blue as a tracer localizing sentinel lymph nodes on cN0 thyroid papillary carci? noma,evaluating the cervical lymphatic metastasis state ,and selecting surgical procedure,has the guid? ing significance.

    Application of 3D endoscopic thyroidectomy via breast approach in primary hospital
    LI Weiqi, YUAN Jie,LIU Hongya,QI Fujian,JIAN Yongping,WANG Zhijun.
    2017, 17(02):  205-208.  DOI: 10.3969/j.issn.1009-976X.2017.02.018
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    Objective To evaluate the efficacy for subtotal thyroidectomy from transvesical approach in primary hospital. Methods The clinical data of thyroid surgery patients in the Fifth Affiliated Hospital of Southern Medical University from September ,2013 to April ,2016 were analyzed. The patients underwent open surgery(open group,n=20),2D endoscopic surgery(2D group,n=20)and 3D endoscopic surgery (3D group ,n=20),respectively. The clinical data of thyroid surgery patients in Zhujiang Hospital were analyzed in same period and 30 cases were in each group. The operative time, intraoperative blood loss,postoperative drainage volume,postoperative hospital stay,complications,total operative complications,hospital costs and hospital data differences were compared between the three groups. Results The time of operation was 69.21±11.6 min in 2D group,56.22±6.57 min in 2D group and 40.93±10.6 min in open group(F=41.64,P<0.05). The hospitalization cost of secondary hospital was 9733±1410 yuan,the cost of tertiary hospital was 11511±1749,and the cost of hospital was statistically different(t=-3.41,P<0.05). The intraoperative blood loss,the postoperative drainage volume and the postoperative hospitalization were not significantly different. Conclusion Endoscopic subtotal thyroidectomy via a breast approach is safe ,effective,economical and practical as conventional surgery in primary hospital. The selection of 2D or 3D systems was based on the amount of operation annually.

    Clinical application and comparison of four different materials on rib fracture internal fixation
    GAO Xiaotian,LIANG Ke,XIE Zhanqiang,ZHANG Zhanfei.
    2017, 17(02):  209-212.  DOI: 10.3969/j.issn.1009-976X.2017.02.019
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    Objective To compare the clinical application efficacy of four internal fixation materials (steel wire,absorbable intramedullary nail,memory embracing fixator,and steel plate)on rib fracture. Methods A total of 83 rib fracture patients were included in this study. Of them ,11 cases were performed internal fixed with steel wire material ,34 patients with absorbable intramedullary nail ,25 patients with memory alloy embracing fixator ,and 13 patients with steel plate. The cost,operation time,complications and pain were compared among the four groups. Results The patients in steel wire group was characterized of a simple operation ,lower cost,but poor broken end reduction and firmness, and a non?absorbable material. Absorbable intramedullary nail group showed a higher cost ,poor restoration of broken ends and fastness,but completely absorbable material. Memory embracing fixator group had vantage to better restoration of broken ends and fastness degree with complex operation and higher cost The last steel plate group was easy to use ,and had a good restoration of broken ends and fastness degree,but non?absorbable material and X?rays exposure. Conclusion Each own relative merits was found in four different internal fixation materials on rib fracture. We should select appropriate material according to material character,special situation of rib fracture,and hospitalization cost.

    Analysis of clinical effect of customized 3D printing?assisted total knee replacement
    CAO Heng, LIU Mingting,SHI Hui,LIU Kang.
    2017, 17(02):  213-216.  DOI: 10.3969/j.issn.1009-976X.2017.02.020
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    Objective To investigate the clinical effect of customized 3D printing?assisted total knee arthroplasty. Methods Ten patients(cutting auxiliary group)from January,2016 to July,2016 were selected. According to the preoperative CT scan data ,total knee arthroplasty was performed under the help of customized 3D printing. The patients who underwent traditional total knee arthroplasty(TAP) were divided into two groups:the amount of blood loss,the operation time,the complication rate(pros? thetic fracture,dislocation,infection and deep venous thrombosis). The changes of HHS score were com? pared between 2 weeks after operation,1 month after operation and 3 months after operation. The above data were analyzed statistically. Results Compared with the control group,the amount of bleeding,the amount of drainage and the time of operation were less in the former group than in the control group (P<0.05),but the difference was statistically significant(P>0.05). There was no significant difference in the incidence of HHS in the ipsilateral knee between 2 weeks,1 month and 3 months postoperatively. There was no significant difference between the two groups in the incidence of complications Statistical significance(P>0.05). Conclusion In the present study,there was no difference in the incidence of knee joint function and complication in patients with total knee arthroplasty after total 3D arthroplasty. However,the operative time was shortened,postoperative drainage decreased.

    Application value of high viscosity bone cement in the treatment of osteoporotic vertebral compression fractures
    DU Guocong,LI Qizhong,MAO Ping,OU Jiexiong,MENG Gengyao,CHEN Ming.
    2017, 17(02):  217-220.  DOI: 10.3969/j.issn.1009-976X.2017.02.021
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    Objective To investigate the application value of high viscosity bone cement in the treatment of osteoporotic vertebral compression fractures. Methods Thirty?seven patients with OVCFs in our hospital from August 2013 to February 2016 were selected and treated with high viscosity bone cement. The pain intensity,activity,and motor function was evaluated in one day after PVP,1?month, 3?month,half an year and 1 year by VAS ,ODI ,SF?36 and the lateral X?ray film of spine. Results The cobbs abgle,VAS score,ODI score,SF?36 score in one?day after operation,1?month,3?month,half a year,1 years after treatment were significantly improved(all P values less than 0.05). The rate of bone cement leakage was 5.4% . There was no serious complication after operation. Conclusion The clinical curative effect of high viscosity bone cement materials can effectively reduce the incidence of postoperative complications,improve the clinical symptoms of the patients and the quality of life of patients.

    Cancer patients with PICC catheter related bloodstream infection with thrombosis : A case analysis
    MO Chunling,QU Yingying,LUO Shujuan
    2017, 17(02):  221-223.  DOI: 10.3969/j.issn.1009-976X.2017.02.022
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    Objective To analyze the cause and pathogenic process in patients with both catheter? related bloodstream infection and catheter?related large vein thrombosis (CRLVT). Methods The central line?associated bloodstream infection (CLABSI) were investigated from tumor patients with PICC between May 2015 to April 2016. Of 863 case with PICC catheter,two cases were diagnosed as CLABSI combined with thrombosis. Results Management of anticoagulation,thrombolysis,systemic anti infection and symptomatic supportive treatment were performed ,and the infection and bone marrow suppression were controlled ,and the local symptoms subsided. After discharge ,oral anticoagulation therapy was continued for one month,and no special discomfort was observed in follow?up. In the two CLABSI patients,poor compliance,poor health situation,local rash,decreased immunity might be the mainly pathogenic factors. Conclusion CLABSI patients should be paid attention to occurrence of thrombosis. Clinical nursing work should be targeted to avoid the possible risk factors in patients with PICC catheter.

    The development of circulating tumor cells in colorectal cancer
    CHEN Chisheng,CHU Zhonghua.
    2017, 17(02):  226-229.  DOI: 10.3969/j.issn.1009-976X.2017.02.024
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    Although the efficacy of surgery,chemotherapy and radiotherapy in colorectal cancer has been greatly improved in recent years ,15%-20% of patients with colorectal cancer had hepatic me? tastases when they were first diagnosed ,and 60% of the rest experienced metastatic or recurrent tumors. As a focus of clinical research in recent years ,circulating tumor cells have the tremendous potential in evaluating curative effect and prognosis. This article reviews the separation enrichment techniques ,detec? tion techniques and clinical significance of circulating tumor cells in colorectal cancer.
    Progress of biomarkers of hepatocellular carcinoma
    LUO Qijie,HU Zemin
    2017, 17(02):  230-235.  DOI: 10.3969/j.issn.1009-976X.2017.02.025
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    Hepatocellular Carcinoma(HCC)is the world′s fifth malignant tumor. Most patients have been in advanced stage and missed the best treatment timing when they are diagnosed. The effective biomarkers are important for the early diagnosis ,the monitoring of tumor progression ,judgement of curative effect,recurrence and survival rate of judgement. This article reviewed the several biomarkers which have been found in the past few years.

    Research progress of epiduralscar after lumbars pinesurgery
    YE Weikang,FANG Qingmin,WANG Dawei,ZHANG Daijie.
    2017, 17(02):  236-239.  DOI: 10.3969/j.issn.1009-976X.2017.02.026
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    Lumbar disc herniation in patients with laminectomy or discectomy after lower limb remained intractable pain symptoms. These symptoms of discomfort may be acquired by postoperative spi? nal stenosis,adjacent segment degeneration,intervertebral disc and lumbar spondylolisthesis recurrence caused by epidural scar and other reasons. Among them,the epidural scar(nerve root adhesion,epidural fibrosis)may account for 20% to 36%. Some scholars have studied how to reduce the epidural scar mate? rials or Methods,but also some scholars committed to the treatment of epidural scar after surgery. This paper made a review on the research progress of epidural scar.
    Significance of inflammatory factors in elderly patients with hip surgery
    FEI Jianping.
    2017, 17(02):  240-243.  DOI: 10.3969/j.issn.1009-976X.2017.01.027
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    The release of inflammatory factors after surgery and trauma were closely related to the postoperative outcome. In this paper ,the current status of perioperative inflammatory response in elderly hip surgery such as the expression of inflammatory factors in elderly patients undergoing hip surgery. Uncontrolled inflammatory response and related complications and the application of inflammatory control theory in hip surgery were explained. At present ,the experimental study or clinical practice have been confirmed those what can effectively inhibit the release of inflammatory factors ,improve the tissue and organ ischemia reperfusion damage such as drugs ,techniques and Methods.
    Problems and countermeasures of surgical clinical internship for overseas medical students
    AI Wenjia,LIN Shaomang,ZHANG Zhihui,XIAO Jianbin,LI qiang,LI Yangyong,WANG Junwei.
    2017, 17(02):  244-246.  DOI: 10.3969/j.issn.1009-976X.2017.02.028
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    The paper is to sum up the teaching experience according to the problems of overseas medical students in the clinical practice teaching. Following issues need to be discussed. The first is to strengthen bilingual teaching of teachers and students ,and promote the professional quality of teachers. The next is to establish some cases of typical surgical diseases ,which to create nearly true environment of diagnosis and treatment,operation opportunity. The third is to improve the teaching system ,the direc? tion and content of assessment. These are the keys to promote the quality of clinical practice teaching for overseas medical students.
    Comparison of the efficacy of two visiting systems in relieving ICU syndrome in awake patients with tracheal intubation
    YU Xinying,WANG Haiqin,YAO Xiaohong
    2017, 17(02):  247-249.  DOI: 10.3969/j.issn.1009-976X.2017.02.029
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    Objective To compare and analyze the efficacy of two visiting systems in relieving ICU syndrome in patients with tracheal intubation. Methods 120 cases of patients with tracheal intubation in our hospital From January 2016 to May were divided into observation group and control group , each 60 cases. The control group followed the current visitation system ,in which one to four family num? bers were only permitted a visit for 20 minutes every day at 4 pm according to the bed number. The obser? vation group allowed more visiting frequency in a stable condition according to an appointment system. The occurrence of ICU syndrome,weaning from mechanical ventilation,reintubation after weaning,ICU nursing service quality satisfaction were observed and compared between two groups. Meanwhile ,the nos? ocomial infection were monitored. Results There were significant differences in the incidence of ICU syndrome,weaning rate,reintubation rate,nursing service quality satisfaction between the two groups (all P values<0.05). The ventilator?associated pneumonia showed no significant difference compared to the same period last year in two groups. Conclusion The management by raising the number of family visits can effectively prevent tracheal intubation in patients with ICU syndrome. As long as the strict im? plementation of disinfection and isolation measures ,visitation increased without increasing the hospital infection.
    Analysis of prevention and nursing effects of hypostatic pneumonia
    HUANG Lixia
    2017, 17(02):  250-252.  DOI: 10.3969/j.issn.1009-976X.2017.02.030
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    Objective To analyze the prevention and nursing of hypostatic pneumonia. Methods Seventy?five patients with long?term bed rest or treatment were selected from May 2016 to November 2016,which were divided into observation group(39 cases)and the control group(36 cases) according to the different nursing Methods. The control group was given regular nursing care. The obserua? tion group were performed more reasonable nursing care ,including oral care,wet airway nursing and health education. The incidence of hypostatic pneumonia in the two groups was observed. The total hospi? talization time of the two groups was also recorded. Results The incidence of hypostatic pneumonia in the observation group was lower than that in the control group (7.69% vs 25% ,P<0.05). The nursing satisfaction in observation group was increased than in control group(P<0.05). Conclusion For the pre? vention and care of hypostatic pneumonia need to increase the intake of nutrients and water intake , strengthen behavioral and psychological intervention ,so as to promote the rehabilitation of patients ,but also makes patients and their families are increasingly satisfied with the work of nursing.
    The clinical experience of table tennis?nose fixed Sengstaken?Blakemore tube in the treatment of ruptured esophageal gastric varices bleeding
    LI Minjiao,ZHONG Xiuqiong,LIANG Qiaoling
    2017, 17(02):  253-254.  DOI: 10.3969/j.issn.1009-976X.2017.02.031
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    Objective To explore the clinical experience of the multiple nursing intervention and the table tennis?nose fixed Sengstaken?Blakemore tube in the treatment of the esophageal and gastric varices bleeding. Methods Retrospective analysis in January 2010 to December 2015 was performed in 50 patients with the vein varicose rupture hemorrhage at esophagus stomach bottom. After the success of Sengstaken?Blakemore tube catheter,the modified table tennis?nasal fixation was used for hemostasis. Results Of 50 patients,47 patients were successful catheter placement ,the failure of the catheter in 3 cases. There was no obvious nasal compression injury and abnormal performance such as trouble breath? ing. Conclusion The improved table tennis?nose fixed Sengstaken?Blakemore tube is effective ,and the operation is simple,easy to care,and with high patient acceptance.