Abstract:

 Objective To investigate the expression of NEK9 in hepatocellular carcinoma （HCC）tissues and its correlation with clinical pathological parameters. Methods Sixty cases of primary HCC tissues, paired normal tissues and their clinical data were collected in our hospital during 2010 to 2011. The mRNA level expression of NEK9 was determined by qRT-PCR. The protein expression of NEK9 was detected by immunohistochemical staining. Then the relationship of NEK9 expression and clinical pathological parameters were analyzed. Results Compared to the paired normal tissues, the expression of NEK9 was explored to be much higher in primary HCC tissues, both at mRNA level（25/30） （P<0.05）, and at protein level（65% and 21.7%）（P<0.05）. The expression of NEK9 was found to be correlated with tumor size, TNM stage metastasis and recurrence and tumor?free survival time（P<0.05）. Conclusion The over?expression of NEK9 is concerned with tumor size, TNM stage, metastasis, recurrence and tumor?free survival time, and maybe a potential marker for early diagnosis and prognosis of HCC.

Key words: NEK9, hepatocellular carcinoma, recurrence, metastasis

摘要：

目的 研究基因NEK9在肝细胞癌组织中的表达效应，并探讨其表达量与其临床病理参数的关系。方法 收集从2010~2011年我院60例原发性肝细胞癌患者肝癌组织及其配对癌旁组织，并统计分析其临床病理资料；同时采用荧光定量PCR、免疫组化方法检测肝癌组织和癌旁组织中NEK9基因和蛋白的表达水平，分析NEK9的表达量与其相对应临床病理参数之间的关系。结果 相对于癌旁组织，肝细胞癌组织中基因NEK9的表达量在mRNA水平显著高表达（25/30）（P<0.05），在蛋白质水平亦呈显著高表达（65%及21.7%）（P<0.05）；癌组织中NEK9的表达水平与TNM分期、转移和复发等临床病理参数有相关性（P<0.05）；癌组织中NEK9高表达组患者的术后无瘤生存时间较低表达组明显缩短（P<0.05）。结论 原发性肝细胞癌组织中NEK9的高表达与肿瘤大小、TNM分期、转移和复发密切相关，并有可能成为预测肝癌患者预后的生物学标志物。

关键词: 原发性肝细胞癌, 肿瘤转移, NEK9, 复发