Study of inhibition of dimethyl sulfoxide on morphine-induced thermal hyperalgesia

doi:j.issn.1009-976X.2013.06.015

Abstract

Abstract: 【Abstract】 Objective To explore the influence of dimethyl sulfoxide (DMSO) on morphine-induced thermal hyperalgesia and the expression of tumor necrosis factor-alpha (TNF-α) in the spinal cord dorsal horn. Methods Thirty-two SD rats were randomly divided into 4 groups, group A (saline + morphine group), group B (10% DMSO 5 mL/kg + morphine group), group C (10% DMSO 5 mL/kg + normal saline group) and group D (saline group). In each group, morphine or normal saline was injected subcutaneously 30 min after I.P normal saline or DMSO at 3 PM respectively. Paw withdrawal thermal latency (PWTL) were measured at 1 day before injection and 3, 6, 10 days after injection respectively and the expression of TNF alpha on spinal cord dorsal horn was detected. Results Compared with 1 day before injection, the PWTL of group A became shortened from 3 days after injection, and it further shortened at 10 day after injection respectively (P<0.01). There were significant differences in the shortening of PWTL between group A and group B 6 and 10 days after injection. The spinal dorsal horn TNF alpha expression of group A was higher than other groups (P<0.01). TNF alpha expression of group B were lower than which of group A and higher than which of group D. Conclusion DMSO can reduce morphine-induced thermal hyperalgesia by inhibiting spinal dorsal horn TNF alpha generation.

Key words: Morphine, Tumor necrosis factor, Hyperalgesia, Dimethyl sulfoxide

摘要： 【摘要】目的观察二甲基亚砜(DMSO)对吗啡诱导的热痛觉过敏及其对脊髓背角肿瘤坏死因子(TNF-α)表达的影响。方法 32只SD大鼠随机分为4组,A组(生理盐水+吗啡组)、B组(10%DMSO 5 mL/kg+吗啡组),C组(10%DMSO 5 mL/kg+生理盐水组)和D组(生理盐水组)。各组大鼠分别每d3PM腹腔注射生理盐水或DMSO,30 min后皮下注射吗啡或生理盐水。观察注药前1 d,注药后3 d、6 d、10 d大鼠热刺激撤足潜伏期的变化及脊髓背角TNF?鄄α表达情况。结果与注药前相比,A组注药后3 d开始热刺激撤足潜伏期缩短,10 d进一步缩短(P<0.01)。与A组比较,B组注药后6 d、10 d热刺激撤足潜伏期延长有统计学意义(P<0.01)。A组脊髓背角TNF?鄄α表达较其它组增高(P<0.01)。B组TNF?鄄α的表达较A组低,较D组高有统计学意义(P<0.01)。结论 DMSO可能通过抑制脊髓背角TNF?鄄α的产生而减轻吗啡诱导的热痛觉过敏。

关键词: 吗啡, 肿瘤坏死因子, 痛觉过敏, 二甲基亚砜

CLC Number:

R614

Du Sujuan,Yang Tao, Chen Ruixia, Ye Xijiu. Study of inhibition of dimethyl sulfoxide on morphine-induced thermal hyperalgesia[J]. Lingnan Modern Clinics in Surgery, 2013, 13(06): 521-524.

杜素娟杨涛陈瑞霞叶西就. 二甲基亚砜抑制吗啡诱导热痛觉过敏的实验研究[J]. 岭南现代临床外科, 2013, 13(06): 521-524.

References

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