Abstract: Objective To investigate the protective effect and potential mechanism of colonic flap reconstruction on colonic tissue in rats with short bowel syndrome （SBS） by upregulating Wnt3a and β-catenin expression. Methods Rats were randomly divided into sham operation （Sham）, SBS model （Model）, and colonic flap reconstruction treatment （Surgery） groups, with 10 rats in each group. Serum Wnt3a levels were detected by ELISA. The mRNA and protein expression of Wnt3a and β-catenin in colonic tissue were analyzed by qPCR and Western blot. Immunohistochemistry was used to observe the expression and distribution of α-amylase and bile salt transport protein in proximal and distal epithelial cells of the colonic flap. Immunohistochemistry was also used to detect the expression and localization of Wnt3a, β-catenin, α-amylase, and bile salt transport protein in colonic tissue. Results Compared with the Sham group, there was no significant difference in serum Wnt3a levels in the Model group （P>0.05）, while Wnt3a levels in the Surgery group were significantly higher than those in the Sham and Model groups （P<0.05）. Compared with the Sham group, the mRNA and protein expression of Wnt3a and β-catenin in colonic tissue were significantly downregulated in the Model group （P<0.05）. The expression of Wnt3a and β-catenin in the Surgery group was significantly higher than that in the Model group （P<0.05）. Immunohistochemical staining showed that there were differences in the expression and distribution of α-amylase and bile salt transport protein in proximal and distal epithelial cells of the colonic flap. Conclusion Colonic flap reconstruction can alleviate pathological changes in SBS rats to a certain extent by upregulating the expression of Wnt3a and β-catenin, which may be one of the potential mechanisms of its therapeutic effect.

Key words: short bowel syndrome, colonic flap reconstruction, Wnt3a, β-catenin, epithelial cells

摘要： 目的 探讨结肠瓣膜成形术通过上调Wnt3a和β-catenin表达对短肠综合征（SBS）大鼠结肠组织的保护作用及其潜在机制。方法 将大鼠随机分为假手术组（Sham）、SBS模型组（Model）和结肠瓣膜成形术治疗组（Surgery）,每组10只。采用ELISA检测血清Wnt3a水平;采用qPCR和Western blot分析结肠组织中Wnt3a和β-catenin的mRNA和蛋白表达;采用免疫组化观察α-淀粉酶和胆盐转运蛋白在结肠瓣膜中的表达和分布;采用免疫组化检测Wnt3a、β-catenin、α-淀粉酶和胆盐转运蛋白在结肠组织中的表达和定位。结果 与Sham组相比,Model组血清Wnt3a水平无显著差异（P>0.05）,而Surgery组Wnt3a水平显著高于Sham组和Model组（P<0.05）。与Sham组相比,Model组结肠组织中Wnt3a和β-catenin的mRNA和蛋白表达显著下调（P<0.05）;Surgery组Wnt3a和β-catenin表达显著高于Model组（P<0.05）。免疫组化染色显示,α-淀粉酶和胆盐转运蛋白在结肠瓣膜近端和远端上皮细胞中的表达和分布存在差异。结论 结肠瓣膜成形术可通过上调Wnt3a和β-catenin的表达,在一定程度上缓解SBS大鼠的病理改变,这可能是其发挥治疗作用的潜在机制之一。

关键词: 短肠综合征, 结肠瓣膜成形术, Wnt3a, β-catenin, 上皮细胞