关键词:  壳聚糖, MUC1, 基因疫苗, 胰腺癌

Abstract:

【Abstract】Objective To study the effects of chitosan as a gene delivery carrier combined with MUC1 gene vaccine for inducing specific humoral and cellular immune responses in mice, whereby to provide the preliminary experimental evidence for the immunotherapy in pancreatic cancer. Methods Thirty mice were divided into 3 groups(n=10, each group), which were injected with chitosan-pDC316-MUC1 plasmid, pDC316-MUC1 plasmid or empty pDC316 plasmid, respectively. ELISA method was used to detect the levels of anti-MUC1 antibody in serum. LDH release assay was used to detect the cytotoxicity of CTL for Capan-2 cells. Results Compared with the other two groups, the levels of anti-MUC1 antibody and the killing rates of CTL for Capan-2 cells in the chitosan-pDC316-MUC1 plasmid group were significantly higher (P<0.05). Conclusion Chitosan combined with MUC1 gene vaccine can induce specific humoral and cellular immune responses in mice effectively, which provides preliminary experimental groundwork for the immunotherapy in pancreatic cancer.

Key words: Chitosan, MUC1, Gene vaccine, Pancreatic cancer